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c mouse antibody against βactin  (Cell Signaling Technology Inc)


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    Structured Review

    Cell Signaling Technology Inc c mouse antibody against βactin
    C Mouse Antibody Against βactin, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 96/100, based on 1136 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/c+mouse+antibody+against+%CE%B2actin/beta-Actin+Mouse+mAb/pmc06115445__41598_2018_31138_MOESM1_ESM-28-50-55
    Average 96 stars, based on 1136 article reviews
    c mouse antibody against βactin - by Bioz Stars, 2026-09
    96/100 stars

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    Blocking Assay:

    Article Title: Development of a Novel 3D Tumor-tissue Invasion Model for High-throughput, High-content Phenotypic Drug Screening
    Article Snippet: Samples containing 30 μg of protein were loaded onto a 4-20% TrisHCl pre-cast gels (Bio-Rad, Hercules, CA) and transferred onto Trans-Blot Turbo Midi Nitrocellulose membranes (Bio-Rad). .. After blocking in SEA BLOCK Blocking Buffer (Thermo Fisher Scientific) overnight at 4° C, the membranes were incubated with mouse antibodies against β-catenin (Cell Signaling Technology, 1:1000), E-cadherin (Cell Signaling Technology, 1:1000), ZO-1 (Cell Signaling Technology, 1:1000), N-cadherin (Santa Cruz Biotechnology, 1:1000), and vimentin (BD Biosciences; 1:1000) overnight at 4° C. Mouse antibody against βactin (Cell Signaling Technology, 1:1000) was used as a loading control. .. Membranes were then washed in 1X PBS with 0.05% Tween-20 (Sigma-Aldrich) and incubated for two hours at room temperature with horseradish-peroxidase–conjugated IRDye 800CW anti-mouse secondary antibody (LI-COR Biosciences, Lincoln, NE; 1:10000).

    Incubation:

    Article Title: Development of a Novel 3D Tumor-tissue Invasion Model for High-throughput, High-content Phenotypic Drug Screening
    Article Snippet: Samples containing 30 μg of protein were loaded onto a 4-20% TrisHCl pre-cast gels (Bio-Rad, Hercules, CA) and transferred onto Trans-Blot Turbo Midi Nitrocellulose membranes (Bio-Rad). .. After blocking in SEA BLOCK Blocking Buffer (Thermo Fisher Scientific) overnight at 4° C, the membranes were incubated with mouse antibodies against β-catenin (Cell Signaling Technology, 1:1000), E-cadherin (Cell Signaling Technology, 1:1000), ZO-1 (Cell Signaling Technology, 1:1000), N-cadherin (Santa Cruz Biotechnology, 1:1000), and vimentin (BD Biosciences; 1:1000) overnight at 4° C. Mouse antibody against βactin (Cell Signaling Technology, 1:1000) was used as a loading control. .. Membranes were then washed in 1X PBS with 0.05% Tween-20 (Sigma-Aldrich) and incubated for two hours at room temperature with horseradish-peroxidase–conjugated IRDye 800CW anti-mouse secondary antibody (LI-COR Biosciences, Lincoln, NE; 1:10000).

    Control:

    Article Title: Development of a Novel 3D Tumor-tissue Invasion Model for High-throughput, High-content Phenotypic Drug Screening
    Article Snippet: Samples containing 30 μg of protein were loaded onto a 4-20% TrisHCl pre-cast gels (Bio-Rad, Hercules, CA) and transferred onto Trans-Blot Turbo Midi Nitrocellulose membranes (Bio-Rad). .. After blocking in SEA BLOCK Blocking Buffer (Thermo Fisher Scientific) overnight at 4° C, the membranes were incubated with mouse antibodies against β-catenin (Cell Signaling Technology, 1:1000), E-cadherin (Cell Signaling Technology, 1:1000), ZO-1 (Cell Signaling Technology, 1:1000), N-cadherin (Santa Cruz Biotechnology, 1:1000), and vimentin (BD Biosciences; 1:1000) overnight at 4° C. Mouse antibody against βactin (Cell Signaling Technology, 1:1000) was used as a loading control. .. Membranes were then washed in 1X PBS with 0.05% Tween-20 (Sigma-Aldrich) and incubated for two hours at room temperature with horseradish-peroxidase–conjugated IRDye 800CW anti-mouse secondary antibody (LI-COR Biosciences, Lincoln, NE; 1:10000).



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    96
    Cell Signaling Technology Inc c mouse antibody against βactin
    C Mouse Antibody Against βactin, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/c+mouse+antibody+against+%CE%B2actin/beta-Actin+Mouse+mAb/pmc06115445__41598_2018_31138_MOESM1_ESM-28-50-55
    Average 96 stars, based on 1 article reviews
    c mouse antibody against βactin - by Bioz Stars, 2026-09
    96/100 stars
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